A radiometric method for predicting effectiveness of chemotherapeutic agents in leprosy Academic Article Article uri icon

Overview

MeSH Major

  • Antibodies, Monoclonal, Murine-Derived
  • Lymphoma, Follicular
  • Radioimmunotherapy
  • T-Lymphocyte Subsets
  • T-Lymphocytes

abstract

  • A simple radiometric method has been developed for evaluating the effect of drugs on the metabolism of M. lepraemurium. The method is based on the measurement of the 14CO2 produced through bacterial metabolism of acetate-U-14C. Seventeen drugs were tested: bacitracin, cephaloridine, chloramphenicol, cycloserine, dactinomycin, DDS, ethionamide, INH, kanamycin, methenamine mandelate, nitrofurantoin, oxacillin, polymyxin B, rifampicin, streptomycin, sulfadimethoxine and vancomycin. The drugs which caused most marked inhibition were chloramphenicol, INH, ethionamide and nitrofurantoin in order of increasing effectiveness. The radiometric study which is completed in 15 days permits direct study of the drug effect on the metabolism of M. lepraemurium and a more rapid screening of antileprosy drugs than has previously been possible. Currently, these observations are being extended to studies of the structure-activity relationships of antileprosy drugs and the metabolism and drug susceptibility of M. leprae in vitro.

publication date

  • December 1974

Research

keywords

  • Academic Article

Identity

PubMed ID

  • 176120

Additional Document Info

start page

  • 234

end page

  • 8

volume

  • 22

number

  • 5