A rapid method for determining protein kinase phosphorylation specificity. Academic Article uri icon

Overview

MeSH Major

  • Microfluidic Analytical Techniques
  • Peptide Library
  • Protein Interaction Mapping
  • Protein-Serine-Threonine Kinases
  • Sequence Analysis, Protein

abstract

  • Selection of target substrates by protein kinases is strongly influenced by the amino acid sequence surrounding the phosphoacceptor site. Identification of the preferred peptide phosphorylation motif for a given kinase permits the production of efficient peptide substrates and greatly simplifies the mapping of phosphorylation sites in protein substrates. Here we describe a combinatorial peptide library method that allows rapid generation of phosphorylation motifs for serine/threonine kinases.

publication date

  • October 2004

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed ID

  • 15782149

Additional Document Info

start page

  • 27

end page

  • 9

volume

  • 1

number

  • 1