The C2 domain of PKCĪ“ is a phosphotyrosine binding domain Academic Article uri icon


MeSH Major

  • Phosphotyrosine
  • Protein Kinase C


  • In eukaryotic cells, the SH2 and PTB domains mediate protein-protein interactions by recognizing phosphotyrosine residues on target proteins. Here we make the unexpected finding that the C2 domain of PKCdelta directly binds to phosphotyrosine peptides in a sequence-specific manner. We provide evidence that this domain mediates PKCdelta interaction with a Src binding glycoprotein, CDCP1. The crystal structure of the PKCdelta C2 domain in complex with an optimal phosphopeptide reveals a new mode of phosphotyrosine binding in which the phosphotyrosine moiety forms a ring-stacking interaction with a histidine residue of the C2 domain. This is also the first example of a protein Ser/Thr kinase containing a domain that binds phosphotyrosine.

publication date

  • April 22, 2005



  • Academic Article



  • eng

Digital Object Identifier (DOI)

  • 10.1016/j.cell.2005.02.019

PubMed ID

  • 15851033

Additional Document Info

start page

  • 271

end page

  • 80


  • 121


  • 2