L-arginine regulates neuronal nitric oxide synthase production of superoxide and hydrogen peroxide. Academic Article uri icon

Overview

MeSH

  • Animals
  • Dose-Response Relationship, Drug
  • Nitric Oxide Synthase Type I
  • Rats

MeSH Major

  • Arginine
  • Hydrogen Peroxide
  • Nerve Tissue Proteins
  • Nitric Oxide Synthase
  • Superoxides

abstract

  • Tetrahydrobiopterin (H(4)B) in the absence of L-arginine has been shown to be an important factor in promoting the direct formation of hydrogen peroxide (H(2)O(2)) at the expense of superoxide (O(2)(*-)) by neuronal nitric oxide synthase (NOS1) [Rosen GM, Tsai P, Weaver J, Porasuphatana S, Roman LJ, Starkov AA, et al. Role of tetrahydrobiopterin in the regulation of neuronal nitric-oxide synthase-generated superoxide. J Biol Chem 2002;277:40275-80]. Based on these findings, it is hypothesized that L-arginine also shifts the equilibrium between O(2)(*-) and H(2)O(2). Experiments were designed to test this theory. As the concentration of L-arginine and N(omega)-hydroxyl-L-arginine increases, the rate of NADPH consumption for H(4)B-bound NOS1 decreased resulting in lower rates of both O(2)(*-) and H(2)O(2) generation, while increasing the rate of nitric oxide (*NO) production. At saturating concentrations of L-arginine or N(omega)-hydroxyl-L-arginine (50microM), NOS1 still produced O(2)(*-) and H(2)O(2). Both L-arginine and N(omega)-hydroxyl-L-arginine have greater impact on the rate of generation of O(2)(*-) than on H(2)O(2).

publication date

  • March 15, 2005

has subject area

  • Animals
  • Arginine
  • Dose-Response Relationship, Drug
  • Hydrogen Peroxide
  • Nerve Tissue Proteins
  • Nitric Oxide Synthase
  • Nitric Oxide Synthase Type I
  • Rats
  • Superoxides

Research

keywords

  • Journal Article

Identity

Language

  • eng

Digital Object Identifier (DOI)

  • 10.1016/j.bcp.2004.12.009

PubMed ID

  • 15748708

Additional Document Info

start page

  • 971

end page

  • 979

volume

  • 69

number

  • 6