A testicular antigen aberrantly expressed in human cancers detected by autologous antibody screening Article Conference Paper uri icon

Overview

MeSH Major

  • Adenoma, Oxyphilic
  • Carcinoma, Renal Cell
  • Extracellular Matrix Proteins
  • Kidney Neoplasms
  • Nerve Tissue Proteins

abstract

  • Serological analysis of recombinant cDNA expression libraries (SEREX) using tumor mRNA and autologous patient serum provides a powerful approach to identify immunogenic tumor antigens. We have applied this methodology to a case of esophageal squamous cell carcinoma and identified several candidate tumor targets. One of these, NY-ESO-1, showed restricted mRNA expression in normal tissues, with high-level mRNA expression found only in testis and ovary tissues. Reverse transcription-PCR analysis showed NY-ESO-1 mRNA expression in a variable proportion of a wide array of human cancers, including melanoma, breast cancer, bladder cancer, prostate cancer, and hepatocellular carcinoma. NY-ESO-1 encodes a putative protein of Mr 17,995 having no homology with any known protein. The pattern of NY-ESO-1 expression indicates that it belongs to an expanding family of immunogenic testicular antigens that are aberrantly expressed in human cancers in a lineage-nonspecific fashion. These antigens, initially detected by either cytotoxic T cells (MAGE, BAGE, GAGE-1) or antibodies [HOM-MEL-40(SSX2), NY-ESO-1], represent a pool of antigenic targets for cancer vaccination.

publication date

  • March 4, 1997

Research

keywords

  • Conference Paper

Identity

Digital Object Identifier (DOI)

  • 10.1073/pnas.94.5.1914

PubMed ID

  • 9050879

Additional Document Info

start page

  • 1914

end page

  • 8

volume

  • 94

number

  • 5