Studies of the Escherichia coli Rsd-σ70 complex Academic Article Article uri icon

Overview

MeSH Major

  • Bacteriological Techniques
  • Genome, Bacterial
  • High-Throughput Nucleotide Sequencing
  • Molecular Diagnostic Techniques

abstract

  • Escherichia coli Rsd protein was previously identified on the basis of its binding to the RNA polymerase sigma(70) subunit. The Rsd-sigma(70) complex has been studied using different methods. Our data show that Rsd associates with sigma(70) to form a complex with a stoichiometry of 1:1. Alanine scanning and deletion mutagenesis were used to locate regions of sigma(70) that are required for the formation of the Rsd-sigma(70) complex.

publication date

  • January 16, 2004

Research

keywords

  • Academic Article

Identity

Digital Object Identifier (DOI)

  • 10.1016/j.jmb.2003.11.004

PubMed ID

  • 14687566

Additional Document Info

start page

  • 685

end page

  • 92

volume

  • 335

number

  • 3