Partial purification and characterization of human γ (immune) interferon Academic Article uri icon

Overview

MeSH Major

  • Interferons

abstract

  • Human gamma (immune) interferon (IFN-gamma) was produced in lymphocyte cultures stimulated with a phorbol ester (12-O-tetradecanoylphorbol 13-acetate) and purified phytohemagglutinin. Physicochemical analysis showed that human IFN-gamma is a glycoprotein with an isoelectric point around 8.6 and an apparent molecular weight of 58,000 +/- 3000. A purification process for IFN-gamma was developed consisting of sequential chromatographic separations on controlled-pore glass, concanavalin A-Sepharose, and Bio-Gel P-200. This purification process resulted in an increase in specific activity from about 10(4) (crude culture fluid) to an estimated 10(7) units per mg of protein with a cumulative recovery of about 40% of the IFN activity.

publication date

  • January 1981

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed Central ID

  • PMC319179

PubMed ID

  • 6165014

Additional Document Info

start page

  • 1601

end page

  • 5

volume

  • 78

number

  • 3 I