Protective immunity evoked against anthrax lethal toxin after a single intramuscular administration of an adenovirus-based vaccine encoding humanized protective antigen. Academic Article uri icon

Overview

MeSH

  • Animals
  • Bacillus anthracis
  • Codon
  • Dose-Response Relationship, Drug
  • Female
  • Humans
  • Immunoglobulin G
  • Injections, Intramuscular
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Time Factors
  • Vaccines, Synthetic

MeSH Major

  • Adenoviridae
  • Anthrax Vaccines
  • Antibodies, Bacterial
  • Antigens, Bacterial
  • Bacterial Toxins

abstract

  • Because of the need to develop a vaccine to rapidly protect the civilian population in response to a bioterrorism attack with Bacillus anthracis, we designed AdsechPA, a replication-deficient human serotype 5 adenovirus encoding B. anthracis protective antigen (PA) with codons optimized for expression in mammalian cells. With a single intramuscular administration to mice of 10(9) particle units of AdsechPA, a dose that can be scaled to human use, anti-PA antibodies were evoked more rapidly and at a higher level than with a single administration of the new U.S. military recombinant PA/Alhydrogel vaccine. Importantly, AdsechPA afforded approximately 2.7-fold more protection than the recombinant PA vaccine against B. anthracis lethal toxin challenge 4 weeks after a single vaccination. Even at 11 days postvaccination, AdsechPA provided some survival benefit, whereas the rPA/Alhydrogel vaccine provided none. In the context that equivalent human doses of Ad vectors have already been demonstrated to be safe in humans, a single administration of AdsechPA may provide the means to rapidly protect the civilian population against B. anthracis in response to a bioterrorism attack.

publication date

  • November 20, 2003

has subject area

  • Adenoviridae
  • Animals
  • Anthrax Vaccines
  • Antibodies, Bacterial
  • Antigens, Bacterial
  • Bacillus anthracis
  • Bacterial Toxins
  • Codon
  • Dose-Response Relationship, Drug
  • Female
  • Humans
  • Immunoglobulin G
  • Injections, Intramuscular
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Time Factors
  • Vaccines, Synthetic

Research

keywords

  • Journal Article

Identity

Language

  • eng

Digital Object Identifier (DOI)

  • 10.1089/104303403322542310

PubMed ID

  • 14633409

Additional Document Info

start page

  • 1673

end page

  • 1682

volume

  • 14

number

  • 17