Cell surface antigens of human malignant melanoma: Definition of six antigenic systems with mouse monoclonal antibodies Academic Article Article uri icon

Overview

MeSH Major

  • Antigens, Neoplasm
  • Genetic Therapy
  • HLA-A2 Antigen
  • Immunologic Factors
  • Immunotherapy, Active
  • Interleukin-2
  • Melanoma
  • Recombinant Fusion Proteins
  • Tumor Cells, Cultured

abstract

  • Eighteen mouse monoclonal antibodies were selected for reactivity with cell surface antigens of the immunizing human melanoma cell line SK-MEL-28. Six distinct antigenic systems were defined by direct serological assays and absorption tests with a panel of 41 cell lines derived from normal and malignant human tissues. Biochemical analysis indicated that two of the antigens are glycoproteins with molecular sizes of 95,000 and 150,000 daltons (gp95 and gp150). Two other antigenic systems (O5 and the R24 group) are associated with heat-stable molecules having the characteristics of glycolipids. The remaining two antigens (M19 and R8) are heat labile, but molecular characterization has not been possible. Each of the antigenic systems has a distinctive pattern of distribution on various cell types, varying from a broad representation to a more restricted occurrence. O5 appears to be a species antigen, being present on virtually every human cell type tested. gp95, gp150, M19, and R8 are found on a characteristic proportion of melanomas, astrocytomas, and epithelial cancers and on normal kidney cells. The antigen defined by the R24 antibody has the most restricted distribution of all. Reactivity is found with melanomas and astrocytomas, whereas epithelial cell types, fibroblasts, and cells of hematopoietic origin lack R24. Although occurrence of gp95, gp150, M19, and R8 distinguishes a small subset of melanomas not expressing these antigens, R24 is found on all melanoma cells.

publication date

  • December 1980

Research

keywords

  • Academic Article

Identity

Digital Object Identifier (DOI)

  • 10.1073/pnas.77.10.6114

PubMed ID

  • 6934537

Additional Document Info

start page

  • 6114

end page

  • 8

volume

  • 77

number

  • 10 II