Effect of Electron-Transport Inhibitors on the Generation of Reactive Oxygen Species by Pea Mitochondria during Succinate Oxidation Academic Article uri icon

Overview

MeSH Major

  • Breast Neoplasms
  • Estrogens
  • Growth Substances

abstract

  • The effect of inhibitors of the cytochrome pathway and alternative oxidase on the rate of respiration and generation of reactive oxygen species by pea mitochondria was studied. Respiration of mitochondria from pea cotyledons was inhibited by 70-80% by salicylhydroxamate (SHAM). The rate of hydrogen peroxide production by pea cotyledon mitochondria during succinate oxidation was 0.15 nmol/min per mg protein. SHAM considerably accelerated the hydrogen peroxide production. The SHAM-dependent H2O2 production was stimulated by 2 micro M antimycin A and inhibited by 5 mM KCN and 1 micro M myxothiazol. The study of the rate of O2*- generation by pea mitochondria using EPR spin traps and epinephrine oxidation showed that H2O2 accumulation can be accounted for by a significant increase in the rate of O2*- production.

publication date

  • July 2003

Research

keywords

  • Academic Article

Identity

Digital Object Identifier (DOI)

  • 10.1023/A:1025078815819

Additional Document Info

start page

  • 747

end page

  • 51

volume

  • 68

number

  • 7