Variability of antioxidant-related gene expression in the airway epithelium of cigarette smokers. Academic Article uri icon

Overview

MeSH

  • Adult
  • Cluster Analysis
  • Cohort Studies
  • DNA, Complementary
  • Down-Regulation
  • Female
  • Gene Expression Regulation
  • Genetic Predisposition to Disease
  • Genetic Variation
  • Humans
  • Male
  • Middle Aged
  • Nucleic Acid Hybridization
  • Oligonucleotide Array Sequence Analysis
  • Oxidants
  • Oxidation-Reduction
  • Phenotype
  • Pulmonary Disease, Chronic Obstructive
  • RNA, Complementary
  • Up-Regulation

MeSH Major

  • Antioxidants
  • Epithelium
  • Gene Expression
  • Lung
  • Smoking

abstract

  • Cigarette smoking is the major risk factor for developing chronic bronchitis, yet only 15-20% of smokers develop this disorder. Because oxidants are the major mechanism of smoking-induced airway damage, we hypothesized that smoking is associated with upregulation of various antioxidant-related genes in the airway epithelium, but the magnitude of the response shows high inter-individual variability. Microarray analysis was used to assess levels of expression of 44 antioxidant-related genes in four categories (catalase/superoxide dismutase family; glutathione metabolism; redox balance; and pentose phosphate cycle) in bronchoscopy-obtained airway epithelium of matched cohorts (13 current smokers, 9 nonsmokers), none of whom had lung disease. There was minimal variation in gene expression levels within the same individual (right versus left lung or over time), but significant upregulation of 16/44 antioxidant-related genes in smoker epithelium compared with nonsmokers. Subgroups of smokers were identified with clusters of expression levels of antioxidant-related genes. We propose that the antioxidant-related genes demonstrating the most variability in the level of expression in smokers may be useful genetic markers in epidemiologic studies assessing susceptibility to smoking-induced chronic bronchitis.

publication date

  • September 2003

has subject area

  • Adult
  • Antioxidants
  • Cluster Analysis
  • Cohort Studies
  • DNA, Complementary
  • Down-Regulation
  • Epithelium
  • Female
  • Gene Expression
  • Gene Expression Regulation
  • Genetic Predisposition to Disease
  • Genetic Variation
  • Humans
  • Lung
  • Male
  • Middle Aged
  • Nucleic Acid Hybridization
  • Oligonucleotide Array Sequence Analysis
  • Oxidants
  • Oxidation-Reduction
  • Phenotype
  • Pulmonary Disease, Chronic Obstructive
  • RNA, Complementary
  • Smoking
  • Up-Regulation

Research

keywords

  • Journal Article

Identity

Language

  • eng

Digital Object Identifier (DOI)

  • 10.1165/rcmb.2002-0321OC

PubMed ID

  • 12702543

Additional Document Info

start page

  • 331

end page

  • 343

volume

  • 29

number

  • 3 Pt 1