Cotransfection of heme oxygenase-1 prevents the acute inflammation elicited by a second adenovirus. Academic Article uri icon

Overview

MeSH

  • Acute Disease
  • Animals
  • Heme Oxygenase-1
  • Liver
  • Male
  • Membrane Proteins
  • Mice
  • Mice, Inbred C57BL
  • Microscopy, Video
  • Microsomes, Liver
  • Reverse Transcriptase Polymerase Chain Reaction

MeSH Major

  • Adenoviridae
  • Genetic Therapy
  • Genetic Vectors
  • Heme Oxygenase (Decyclizing)
  • Hepatitis
  • Transfection

abstract

  • The acute inflammatory response elicited by adenovirus vectors results in loss of gene expression and tissue injury in the target organ. This acute inflammation is now believed to be the major limiting factor for the use of adenovirus vectors in gene therapy. While exploring the level of acute inflammation caused by the adenovirus encoding the gene for the anti-inflammatory enzyme heme oxygenase-1, we discovered that this adenovirus not only did not elicit acute inflammation, but could prevent the inflammation caused by a second adenovirus. Here we describe a new approach to gene therapy, which uses the encoding of the potent anti-inflammatory enzyme heme oxygenase-1 to prevent early host inflammatory responses normally associated with adenovirus vectors.

publication date

  • September 2003

has subject area

  • Acute Disease
  • Adenoviridae
  • Animals
  • Genetic Therapy
  • Genetic Vectors
  • Heme Oxygenase (Decyclizing)
  • Heme Oxygenase-1
  • Hepatitis
  • Liver
  • Male
  • Membrane Proteins
  • Mice
  • Mice, Inbred C57BL
  • Microscopy, Video
  • Microsomes, Liver
  • Reverse Transcriptase Polymerase Chain Reaction
  • Transfection

Research

keywords

  • Journal Article

Identity

Language

  • eng

Digital Object Identifier (DOI)

  • 10.1038/sj.gt.3302063

PubMed ID

  • 12923561

Additional Document Info

start page

  • 1629

end page

  • 1635

volume

  • 10

number

  • 19