Regulation of blood and lymphatic vascular separation by signaling proteins SLP-76 and Syk Academic Article uri icon


MeSH Major

  • Blood Vessels
  • Enzyme Precursors
  • Fluorescein-5-isothiocyanate
  • Lymphatic System
  • Phosphoproteins
  • Protein-Tyrosine Kinases
  • Signal Transduction


  • Lymphatic vessels develop from specialized endothelial cells in preexisting blood vessels, but the molecular signals that regulate this separation are unknown. Here we identify a failure to separate emerging lymphatic vessels from blood vessels in mice lacking the hematopoietic signaling protein SLP-76 or Syk. Blood-lymphatic connections lead to embryonic hemorrhage and arteriovenous shunting. Expression of slp-76 could not be detected in endothelial cells, and blood-filled lymphatics also arose in wild-type mice reconstituted with SLP-76-deficient bone marrow. These studies reveal a hematopoietic signaling pathway required for separation of the two major vascular networks in mammals.

publication date

  • January 10, 2003



  • Academic Article



  • eng

PubMed Central ID

  • PMC2982679

Digital Object Identifier (DOI)

  • 10.1126/science.1079477

PubMed ID

  • 12522250

Additional Document Info

start page

  • 247

end page

  • 51


  • 299


  • 5604