Control of biochemical reactions through supramolecular RING domain self-assembly Academic Article uri icon


MeSH Major

  • Macromolecular Substances
  • Nuclear Proteins
  • Protein Structure, Tertiary
  • Tumor Suppressor Proteins
  • Ubiquitin-Conjugating Enzymes
  • Ubiquitin-Protein Ligases


  • RING domains act in a variety of unrelated biochemical reactions, with many of these domains forming key parts of supramolecular assemblies in cells. Here, we observe that purified RINGs from a variety of functionally unrelated proteins, including promyelocytic leukemia protein, KAP-1TIF1beta, Z, Mel18, breast cancer susceptibility gene product 1 (BRCA1), and BRCA1-associated RING domain (BARD1), self-assemble into supramolecular structures in vitro that resemble those they form in cells. RING bodies form polyvalent binding surfaces and scaffold multiple partner proteins. Separation of RING bodies from monomers reveals that self-assembly controls and amplifies their specific activities in two unrelated biochemistries: reduction of 5' mRNA cap affinity of eIF4E by promyelocytic leukemia protein and Z, and E3 ubiquitin conjugation activity of BARD1:BRCA1. Functional significance of self-assembly is underscored by partial restoration of assembly and E3 activity of cancer predisposing BRCA1 mutant by forced oligomerization. RING self-assembly creates bodies that act structurally as polyvalent scaffolds, thermodynamically by amplifying activities of partner proteins, and catalytically by spatiotemporal coupling of enzymatic reactions. These studies reveal a general paradigm of how supramolecular structures may function in cells.

publication date

  • November 26, 2002



  • Academic Article



  • eng

PubMed Central ID

  • PMC137729

Digital Object Identifier (DOI)

  • 10.1073/pnas.202608799

PubMed ID

  • 12438698

Additional Document Info

start page

  • 15404

end page

  • 9


  • 99


  • 24