A p5 integration efficiency element mediates Rep-dependent integration into AAVS1 at chromosome 19 Academic Article uri icon

Overview

MeSH Major

  • Chromosomes, Human, Pair 19
  • DNA-Binding Proteins
  • Dependovirus
  • Viral Proteins
  • Virus Integration

abstract

  • Adeno-associated virus (AAV) undergoes site-specific integration into human chromosome 19 through a deletion-substitution mechanism at the well characterized AAVS1 site. We have shown previously that a cis element within the left end of the AAV genome enhances the efficiency of Rep-mediated site-specific integration into chromosome 19 when present in inverted terminal repeat-containing recombinant AAV (rAAV) plasmids. We now demonstrate that a 138-bp cis element, the p5 integration efficiency element (p5IEE), mediates efficient integration. The p5IEE is not only required for efficient site-specific integration, it is also sufficient. Integration mediated by the p5IEE occurs in the absence of the AAV inverted terminal-repeat elements. The data presented in this study demonstrate that the p5IEE is a multifunctional element, serving as the highly regulatable Rep promoter and the primary substrate for targeted integration.

publication date

  • September 17, 2002

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed Central ID

  • PMC129453

Digital Object Identifier (DOI)

  • 10.1073/pnas.182430299

PubMed ID

  • 12221283

Additional Document Info

start page

  • 12381

end page

  • 5

volume

  • 99

number

  • 19