A hereditary nonpolyposis colorectal carcinoma case associated with hypermethylation of the MLH1 gene in normal tissue and loss of heterozygosity of the unmethylated allele in the resulting microsatellite instability-high tumor Academic Article uri icon

Overview

MeSH Major

  • Alleles
  • Colorectal Neoplasms, Hereditary Nonpolyposis
  • DNA Methylation
  • Loss of Heterozygosity
  • Neoplasm Proteins

abstract

  • Fourteen suspected hereditary nonpolyposis colorectal carcinoma cases with microsatellite unstable(microsatellite instability-high; MSI-H) tumors but no germ-line MSH2, MSH6, or MLH1 mutations were examined for hypermethylation of CpG sites in the critical promoter region of MLH1. The methylation patterns were determined using methylation-specific PCR and by sequence analysis of sodium bisulfite-treated genomic DNA. In one case, DNA hypermethylation of one allele was detected in DNA isolated from blood. In the MSI-H tumor from this case, the unmethylated MLH1 allele was eliminated by loss of heterozygosity, and the methylated allele was retained. This biallelic inactivation resulted in loss of expression of MLH1 in the tumor as confirmed by immunohistochemistry. These results suggest a novel mode of germ-line inactivation of a cancer susceptibility gene.

publication date

  • July 15, 2002

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed ID

  • 12124320

Additional Document Info

start page

  • 3925

end page

  • 8

volume

  • 62

number

  • 14