Raf and Akt mediate distinct aspects of sensory axon growth Academic Article Article uri icon

Overview

MeSH Major

  • Biopterin
  • Gene Expression Regulation
  • Inflammation
  • Neuralgia

abstract

  • Nerve growth factor (NGF) induces dramatic axon growth from responsive embryonic peripheral neurons. However, the roles of the various NGF-triggered signaling cascades in determining specific axon morphological features remain unknown. Here, we transfected activated and inhibitory mutants of Trk effectors into sensory neurons lacking the proapoptotic protein Bax. This allowed axon growth to be studied in the absence of NGF, enabling us to observe the contributions of individual signaling mediators. While Ras was both necessary and sufficient for NGF-stimulated axon growth, the Ras effectors Raf and Akt induced distinct morphologies. Activated Raf-1 caused axon lengthening comparable to NGF, while active Akt increased axon caliber and branching. Our results suggest that the different Trk effector pathways mediate distinct morphological aspects of developing neurons.

publication date

  • July 3, 2002

Research

keywords

  • Academic Article

Identity

Digital Object Identifier (DOI)

  • 10.1016/S0896-6273(02)00752-3

PubMed ID

  • 12123609

Additional Document Info

start page

  • 65

end page

  • 76

volume

  • 35

number

  • 1