Mutation profiling of mismatch repair-deficient colorectal cancers using an in Silico genome scan to identify coding microsatellites Academic Article uri icon

Overview

MeSH Major

  • Base Pair Mismatch
  • Colorectal Neoplasms
  • Microsatellite Repeats
  • Mutation

abstract

  • Human colorectal, endometrial, and gastric cancers with defective DNA mismatch repair (MMR) have microsatellite instability, a unique molecular alteration characterized by widespread frameshift mutations of repetitive DNA sequences. We developed "Kangaroo," a bioinformatics program for searches in nucleotide and protein sequence databases, and performed an in silico genome scan for DNA coding microsatellites that may have novel mutations in MMR-deficient cancers. Examination of 29 previously untested coding polyadenines revealed widespread mutations in MMR-deficient colorectal cancers, with the highest frequencies in ERCC5, CASP8AP2, p72, RAD50, CDC25, RECQL1, CBF2, RACK7, GRK4, and DNAPK (range, 10-33%). This algorithm allows comprehensive mutation profiling of MMR-deficient cancers, an important step in understanding the pathogenesis of these neoplasms.

publication date

  • March 2002

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed ID

  • 11888892

Additional Document Info

start page

  • 1284

end page

  • 8

volume

  • 62

number

  • 5