Differentiation of embryonic stem cell lines generated from adult somatic cells by nuclear transfer Academic Article uri icon

Overview

MeSH Major

  • Blastocyst
  • Cell Differentiation
  • Germ Cells
  • Neurons
  • Nuclear Transfer Techniques
  • Stem Cells

abstract

  • Embryonic stem (ES) cells are fully pluripotent in that they can differentiate into all cell types, including gametes. We have derived 35 ES cell lines via nuclear transfer (ntES cell lines) from adult mouse somatic cells of inbred, hybrid, and mutant strains. ntES cells contributed to an extensive variety of cell types, including dopaminergic and serotonergic neurons in vitro and germ cells in vivo. Cloning by transfer of ntES cell nuclei could result in normal development of fertile adults. These studies demonstrate the full pluripotency of ntES cells.

publication date

  • April 27, 2001

Research

keywords

  • Academic Article

Identity

Language

  • eng

Digital Object Identifier (DOI)

  • 10.1126/science.1059399

PubMed ID

  • 11326103

Additional Document Info

start page

  • 740

end page

  • 3

volume

  • 292

number

  • 5517