Solution structure of the A loop of 23S ribosomal RNA. Academic Article uri icon

Overview

MeSH

  • Base Pairing
  • Hydrogen-Ion Concentration
  • Magnetic Resonance Spectroscopy
  • Methylation
  • Models, Molecular
  • Peptides
  • RNA, Transfer

MeSH Major

  • Escherichia coli
  • Nucleic Acid Conformation
  • RNA, Ribosomal, 23S

abstract

  • The A loop is an essential RNA component of the ribosome peptidyltransferase center that directly interacts with aminoacyl (A)-site tRNA. The A loop is highly conserved and contains a ubiquitous 2'-O-methyl ribose modification at position U2552. Here, we present the solution structure of a modified and unmodified A-loop RNA to define both the A-loop fold and the structural impact of the U2552 modification. Solution data reveal that the A-loop RNA has a compact structure that includes a noncanonical base pair between C2556 and U2552. NMR evidence is presented that the N3 position of C2556 has a shifted pKa and that protonation at C2556-N3 changes the C-U pair geometry. Our data indicate that U2552 methylation modifies the A-loop fold, in particular the dynamics and position of residues C2556 and U2555. We compare our structural data with the structure of the A loop observed in a recent 50S crystal structure [Ban, N., Nissen, P., Hansen, J., Moore, P. B. & Steitz, T. A. (2000) Science 289, 905--920; Nissen, P., Hansen, J., Ban, N., Moore, P. B. & Steitz, T. A. (2000) Science 289, 920--930]. The solution and crystal structures of the A loop are dramatically different, suggesting that a structural rearrangement of the A loop must occur on docking into the peptidyltransferase center. Possible roles of this docking event, the shifted pKa of C2556 and the U2552 2'-O-methylation in the mechanism of translation, are discussed.

publication date

  • March 27, 2001

has subject area

  • Base Pairing
  • Escherichia coli
  • Hydrogen-Ion Concentration
  • Magnetic Resonance Spectroscopy
  • Methylation
  • Models, Molecular
  • Nucleic Acid Conformation
  • Peptides
  • RNA, Ribosomal, 23S
  • RNA, Transfer

Research

keywords

  • Journal Article

Identity

Language

  • eng

PubMed Central ID

  • PMC31119

Digital Object Identifier (DOI)

  • 10.1073/pnas.051608498

PubMed ID

  • 11259644

Additional Document Info

start page

  • 3720

end page

  • 3725

volume

  • 98

number

  • 7