CD95 Signaling via Ceramide-rich Membrane Rafts Academic Article uri icon

Overview

MeSH Major

  • Antigens, CD95
  • Ceramides
  • Signal Transduction

abstract

  • Clustering seems to be employed by many receptors for transmembrane signaling. Here, we show that acid sphingomyelinase (ASM)-released ceramide is essential for clustering of CD95. In vitro and in vivo, extracellularly orientated ceramide, released upon CD95-triggered translocation of ASM to the plasma membrane outer surface, enabled clustering of CD95 in sphingolipid-rich membrane rafts and apoptosis induction. Whereas ASM deficiency, destruction of rafts, or neutralization of surface ceramide prevented CD95 clustering and apoptosis, natural ceramide only rescued ASM-deficient cells. The data suggest CD95-mediated clustering by ceramide is prerequisite for signaling and death.

publication date

  • June 8, 2001

Research

keywords

  • Academic Article

Identity

Language

  • eng

Digital Object Identifier (DOI)

  • 10.1074/jbc.M101207200

PubMed ID

  • 11279185

Additional Document Info

start page

  • 20589

end page

  • 96

volume

  • 276

number

  • 23