Distinct roles of class I and class III phosphatidylinositol 3-kinases in phagosome formation and maturation Academic Article uri icon

Overview

MeSH Major

  • Phagocytosis
  • Phagosomes
  • Phosphatidylinositol 3-Kinases

abstract

  • Phagosomes acquire their microbicidal properties by fusion with lysosomes. Products of phosphatidylinositol 3-kinase (PI 3-kinase) are required for phagosome formation, but their role in maturation is unknown. Using chimeric fluorescent proteins encoding tandem FYVE domains, we found that phosphatidylinositol 3-phosphate (PI[3]P) accumulates greatly but transiently on the phagosomal membrane. Unlike the 3'-phosphoinositides generated by class I PI 3-kinases which are evident in the nascent phagosomal cup, PI(3)P is only detectable after the phagosome has sealed. The class III PI 3-kinase VPS34 was found to be responsible for PI(3)P synthesis and essential for phagolysosome formation. In contrast, selective ablation of class I PI 3-kinase revealed that optimal phagocytosis, but not maturation, requires this type of enzyme. These results highlight the differential functional role of the two families of kinases, and raise the possibility that PI(3)P production by VPS34 may be targeted during the maturation arrest induced by some intracellular parasites.

publication date

  • October 2001

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed Central ID

  • PMC2150784

Digital Object Identifier (DOI)

  • 10.1083/jcb.200107069

PubMed ID

  • 11581283

Additional Document Info

start page

  • 19

end page

  • 25

volume

  • 155

number

  • 1