Detection of caspases activation by fluorochrome-labeled inhibitors: Multiparameter analysis by laser scanning cytometry Academic Article Article uri icon

Overview

MeSH Major

  • Cyclins
  • Depsipeptides
  • Enzyme Inhibitors
  • Histone Deacetylase Inhibitors
  • Peptides, Cyclic

abstract

  • The detection of caspase activation combined with cell permeabilization requires exposure of live cells to FLICA followed by their fixation. Cell reactivity with the respective FLICA, under these conditions, identifies the activated caspases and makes it possible to correlate their activation with the cell cycle position and other cell attributes that can be measured only after cell fixation/permeabilization. FLICA can also be used to study intracellular localization of caspases, including their translocation.

publication date

  • May 2001

Research

keywords

  • Academic Article

Identity

Digital Object Identifier (DOI)

  • 10.1002/1097-0320(20010501)44:1<73::AID-CYTO1084>3.0.CO;2-S

PubMed ID

  • 11309811

Additional Document Info

start page

  • 73

end page

  • 82

volume

  • 44

number

  • 1