Thalidomide and its analogues inhibit lipopolysaccharide-mediated induction of cyclooxygenase-2 Academic Article uri icon

Overview

MeSH Major

  • Isoenzymes
  • Lipopolysaccharides
  • Prostaglandin-Endoperoxide Synthases
  • Thalidomide

abstract

  • We investigated the effect of thalidomide, a compound with immunomodulatory and antiangiogenic properties, on lipopolysaccharide (LPS)-mediated induction of cyclooxygenase-2 (Cox-2) and prostaglandin (PG) biosynthesis in murine macrophages. Thalidomide caused a dose-dependent inhibition of LPS-mediated induction of PGE(2) synthesis in RAW 264.7 cells. The induction of Cox-2 protein and mRNA by LPS was also suppressed by thalidomide. Based on the results of nuclear run-off assays and transient transfections, treatment with LPS stimulated Cox-2 transcription, an effect that was unaffected by thalidomide. Thalidomide decreased the stability of Cox-2 mRNA. A series of structural analogues of thalidomide also inhibited LPS-mediated induction of Cox-2 and PGE(2) synthesis. Taken together, these data provide new insights into the antineoplastic and anti-inflammatory properties of thalidomide.

publication date

  • November 28, 2001

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed ID

  • 11705847

Additional Document Info

start page

  • 3349

end page

  • 55

volume

  • 7

number

  • 11