Rapid assessment of adenovirus serum neutralizing antibody titer based on quantitative, morphometric evaluation of capsid binding and intracellular trafficking: population analysis of adenovirus capsid association with cells is predictive of adenovirus infectivity. Academic Article uri icon

Overview

MeSH

  • Biological Transport
  • Cell Line
  • Genetic Therapy
  • Humans
  • Microscopy, Fluorescence

MeSH Major

  • Adenoviridae
  • Antibodies, Viral
  • Capsid

abstract

  • Neutralizing antiviral antibodies are typically detected on the basis of inhibition of viral function, such as propagation of a viral infection or inhibition of viral gene expression. Evidence is presented that anti-adenovirus neutralizing antibodies can be evaluated by analysis of cell-associated capsids or by analysis of intracellular trafficking of the capsids within 1 h after infection. Quantitative analyses of these morphologic parameters represent rapid, broadly applicable, functional assays for the detection of anti-adenovirus neutralizing antibodies.

publication date

  • February 2001

has subject area

  • Adenoviridae
  • Antibodies, Viral
  • Biological Transport
  • Capsid
  • Cell Line
  • Genetic Therapy
  • Humans
  • Microscopy, Fluorescence

Research

keywords

  • Journal Article

Identity

Language

  • eng

PubMed Central ID

  • PMC114056

Digital Object Identifier (DOI)

  • 10.1128/JVI.75.3.1516-1521.2001

PubMed ID

  • 11152523

Additional Document Info

start page

  • 1516

end page

  • 1521

volume

  • 75

number

  • 3