Protein S-nitrosylation: A physiological signal for neuronal nitric oxide Academic Article uri icon


MeSH Major

  • Glutathione
  • Mercaptoethanol
  • Nerve Tissue Proteins
  • Nitric Oxide
  • Nitric Oxide Synthase
  • Nitroso Compounds
  • Proteins
  • S-Nitrosothiols


  • Nitric oxide (NO) has been linked to numerous physiological and pathophysiological events that are not readily explained by the well established effects of NO on soluble guanylyl cyclase. Exogenous NO S-nitrosylates cysteine residues in proteins, but whether this is an important function of endogenous NO is unclear. Here, using a new proteomic approach, we identify a population of proteins that are endogenously S-nitrosylated, and demonstrate the loss of this modification in mice harbouring a genomic deletion of neuronal NO synthase (nNOS). Targets of NO include metabolic, structural and signalling proteins that may be effectors for neuronally generated NO. These findings establish protein S-nitrosylation as a physiological signalling mechanism for nNOS.

publication date

  • February 15, 2001



  • Academic Article



  • eng

Digital Object Identifier (DOI)

  • 10.1038/35055104

PubMed ID

  • 11175752

Additional Document Info

start page

  • 193

end page

  • 7


  • 3


  • 2