Cyclooxygenase-2 is overexpressed in human cervical cancer Academic Article uri icon

Overview

MeSH Major

  • Adenocarcinoma
  • Carcinoma, Adenosquamous
  • Carcinoma, Squamous Cell
  • Isoenzymes
  • Prostaglandin-Endoperoxide Synthases
  • Sarcoma
  • Uterine Cervical Neoplasms

abstract

  • Multiple lines of evidence suggest that cyclooxygenase-2 (COX-2) is an important target for preventing epithelial malignancies. Little is known, however, about the expression of COX-2 in gynecological malignancies. By immunoblot analysis, COX-2 was detected in 12 of 13 cases of cervical cancer but was undetectable in normal cervical tissue. Immunohistochemistry revealed COX-2 in malignant epithelial cells. COX-2 was also expressed in cervical intraepithelial neoplasia. The mechanism by which COX-2 is up-regulated in cervical cancer is unknown. Because the epidermal growth factor (EGF) receptor is commonly overexpressed in cervical cancer, we investigated whether EGF could induce COX-2 in cultured human cervical carcinoma cells. Treatment with EGF markedly induced COX-2 protein, COX-2 mRNA, and stimulated COX-2 promoter activity. The induction of COX-2 by EGF was suppressed by inhibitors of tyrosine kinase activity, phosphatidylinositol 3-kinase, mitogen-activated protein kinase kinase, and p38 mitogen-activated protein kinase. Moreover, overexpressing dominant-negative forms of extracellular signal-regulated kinase 1, c-Jun NH2-terminal kinase, p38, and c-Jun blocked EGF-mediated induction of COX-2 promoter activity. Taken together, these findings suggest that deregulation of the EGF receptor signaling pathway may lead to enhanced COX-2 expression in cervical cancer.

publication date

  • March 12, 2001

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed ID

  • 11234900

Additional Document Info

start page

  • 429

end page

  • 34

volume

  • 7

number

  • 2