Herpesvirus saimiri open reading frame 50 (Rta) protein reactivates the lytic replication cycle in a persistently infected A549 cell line. Academic Article uri icon

Overview

MeSH

  • DNA, Viral
  • Gene Expression Regulation, Viral
  • Humans
  • Tumor Cells, Cultured
  • Virus Latency

MeSH Major

  • Herpesvirus 2, Saimiriine
  • Open Reading Frames
  • Trans-Activators
  • Viral Proteins
  • Virus Activation
  • Virus Replication

abstract

  • Herpesviruses occur in two distinct forms of infection, lytic replication and latent persistence. In this study, we investigated the molecular mechanisms that govern the latent-lytic switch in the prototype gamma-2 herpesvirus, herpesvirus saimiri (HVS). We utilized a persistently HVS-infected A549 cell line, in which HVS DNA is stably maintained as nonintegrated circular episomes, to assess the role of the open reading frame 50 (ORF 50) (Rta) proteins in the latent-lytic switch. Northern blot analysis and virus recovery assays determined that the ORF 50a gene product, when expressed under the control of a constitutively active promoter, was sufficient to reactivate the entire lytic replication cycle, producing infectious virus particles. Furthermore, although the ORF 50 proteins of HVS strains A11 and C488 are structurally divergent, they were both capable of inducing the lytic replication cycle in this model of HVS latency.

publication date

  • April 2001

has subject area

  • DNA, Viral
  • Gene Expression Regulation, Viral
  • Herpesvirus 2, Saimiriine
  • Humans
  • Open Reading Frames
  • Trans-Activators
  • Tumor Cells, Cultured
  • Viral Proteins
  • Virus Activation
  • Virus Latency
  • Virus Replication

Research

keywords

  • Journal Article

Identity

Language

  • eng

PubMed Central ID

  • PMC114895

Digital Object Identifier (DOI)

  • 10.1128/JVI.75.8.4008-4013.2001

PubMed ID

  • 11264393

Additional Document Info

start page

  • 4008

end page

  • 4013

volume

  • 75

number

  • 8