A method for efficient isotopic labeling of recombinant proteins Academic Article uri icon

Overview

MeSH Major

  • Hypertension, Pulmonary
  • Infant, Newborn, Diseases
  • Scleroderma, Systemic

abstract

  • A rapid and efficient approach for preparing isotopically labeled recombinant proteins is presented. The method is demonstrated for 13C labeling of the C-terminal domain of angiopoietin-2, 15N labeling of ubiquitin and for 2H/13C/15N labeling of the Escherichia coli outer-membrane lipoprotein Lpp-56. The production method generates cell mass using unlabeled rich media followed by exchange into a small volume of labeled media at high cell density. Following a short period for growth recovery and unlabeled metabolite clearance, the cells are induced. The expression yields obtained provide a fourfold to eightfold reduction in isotope costs using simple shake flask growths.

publication date

  • June 21, 2001

Research

keywords

  • Academic Article

Identity

Digital Object Identifier (DOI)

  • 10.1023/A:1011254402785

Additional Document Info

start page

  • 71

end page

  • 5

volume

  • 20

number

  • 1