Rme-1 regulates the distribution and function of the endocytic recycling compartment in mammalian cells Academic Article uri icon

Overview

MeSH Major

  • Caenorhabditis elegans Proteins
  • Calcium-Binding Proteins
  • Cell Compartmentation
  • Glycoproteins
  • Membrane Proteins
  • Phosphoproteins
  • Transport Vesicles

abstract

  • RME-1 is an Eps15-homology (EH)-domain protein that was identified in a genetic screen for endocytosis genes in Caenorhabditis elegans. When expressed in a CHO cell line, the worm RME-1 protein and a mouse homologue are both associated with the endocytic recycling compartment. Here we show that expression of a dominant-negative construct with a point mutation near the EH domain results in redistribution of the endocytic recycling compartment and slowing down of transferrin receptor recycling. The delivery of a TGN38 chimaeric protein to the trans-Golgi network is also slowed down. The function of Rme-1 in endocytic recycling is evolutionarily conserved in metazoans as shown by the protein's properties in C. elegans.

publication date

  • June 26, 2001

Research

keywords

  • Academic Article

Identity

Language

  • eng

Digital Object Identifier (DOI)

  • 10.1038/35078543

PubMed ID

  • 11389441

Additional Document Info

start page

  • 567

end page

  • 72

volume

  • 3

number

  • 6