Phagocytosis of wild-type Legionella pneumophila occurs through a wortmannin-insensitive pathway Academic Article uri icon

Overview

MeSH Major

  • Androstadienes
  • Legionella pneumophila
  • Phagocytosis
  • Phosphatidylinositol 3-Kinases

abstract

  • Wild-type Legionella pneumophila grows in human macrophages within a replicative phagosome, avoiding lysosomal fusion, while nonreplicative mutants are killed in lysosomes. Wortmannin, a phosphatidylinositol 3-kinase (PI3K) inhibitor, blocks phagocytosis of an avirulent mutant, but not of wild-type L. pneumophila, without affecting membrane ruffling and actin polymerization. These results show that wild-type and mutant Legionella strains use different entry pathways. They suggest that PI3Ks are involved in phagocytosis of an avirulent L. pneumophila mutant and regulate the ability of microorganisms to generate a replicative phagosome.

publication date

  • August 4, 2001

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed Central ID

  • PMC98612

Digital Object Identifier (DOI)

  • 10.1128/IAI.69.8.5157-5161.2001

PubMed ID

  • 11447198

Additional Document Info

start page

  • 5157

end page

  • 61

volume

  • 69

number

  • 8