HIV gag mRNA transfection of dendritic cells (DC) delivers encoded antigen to MHC class I and II molecules, causes DC maturation, and induces a potent human in vitro primary immune response Academic Article uri icon

Overview

MeSH Major

  • Dendritic Cells
  • Gene Products, gag
  • HIV
  • Histocompatibility Antigens Class I
  • Histocompatibility Antigens Class II
  • RNA, Messenger
  • T-Lymphocyte Subsets
  • Transfection

abstract

  • Dendritic cells (DC) are the major APCs involved in naive T cell activation making them prime targets of vaccine research. We observed that mRNA was efficiently transfected, resulting in superior translation in DC compared with other professional APCs. A single stimulation of T cells by HIV gag-encoded mRNA-transfected DC in vitro resulted in primary CD4(+) and CD8(+) T cell immune responses at frequencies of Ag-specific cells (5-12.5%) similar to primary immune responses observed in vivo in murine models. Additionally, mRNA transfection also delivered a maturation signal to DC. Our results demonstrated that mRNA-mediated delivery of encoded Ag to DC induced potent primary T cell responses in vitro. mRNA transfection of DC, which mediated efficient delivery of antigenic peptides to MHC class I and II molecules, as well as delivering a maturation signal to DC, has the potential to be a potent and effective anti-HIV T cell-activating vaccine.

publication date

  • October 15, 2000

Research

keywords

  • Academic Article

Identity

Language

  • eng

Digital Object Identifier (DOI)

  • 10.4049/jimmunol.165.8.4710

PubMed ID

  • 11035115

Additional Document Info

start page

  • 4710

end page

  • 7

volume

  • 165

number

  • 8