Identification of diverse nerve growth factor-regulated genes by serial analysis of gene expression (SAGE) profiling Academic Article uri icon

Overview

MeSH Major

  • Gene Expression Profiling
  • Gene Expression Regulation
  • Nerve Growth Factor
  • Tyrosine 3-Monooxygenase

abstract

  • Neurotrophic factors such as nerve growth factor (NGF) promote a wide variety of responses in neurons, including differentiation, survival, plasticity, and repair. Such actions often require changes in gene expression. To identify the regulated genes and thereby to more fully understand the NGF mechanism, we carried out serial analysis of gene expression (SAGE) profiling of transcripts derived from rat PC12 cells before and after NGF-promoted neuronal differentiation. Multiple criteria supported the reliability of the profile. Approximately 157,000 SAGE tags were analyzed, representing at least 21,000 unique transcripts. Of these, nearly 800 were regulated by 6-fold or more in response to NGF. Approximately 150 of the regulated transcripts have been matched to named genes, the majority of which were not previously known to be NGF-responsive. Functional categorization of the regulated genes provides insight into the complex, integrated mechanism by which NGF promotes its multiple actions. It is anticipated that as genomic sequence information accrues the data derived here will continue to provide information about neurotrophic factor mechanisms.

publication date

  • September 12, 2000

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed Central ID

  • PMC27040

PubMed ID

  • 10984536

Additional Document Info

start page

  • 10424

end page

  • 9

volume

  • 97

number

  • 19