p53 is associated with cellular microtubules and is transported to the nucleus by dynein Academic Article uri icon

Overview

MeSH Major

  • Active Transport, Cell Nucleus
  • Dyneins
  • Microtubule-Associated Proteins
  • Microtubules
  • Tumor Suppressor Protein p53

abstract

  • Here we show that p53 protein is physically associated with tubulin in vivo and in vitro, and that it localizes to cellular microtubules. Treatment with vincristine or paclitaxel before DNA-damage or before leptomycin B treatment reduces nuclear accumulation of p53 and expression of mdm2 and p21. Overexpression of dynamitin or microinjection of anti-dynein antibody before DNA damage abrogates nuclear accumulation of p53. Our results indicate that transport of p53 along microtubules is dynein-dependent. The first 25 amino acids of p53 contain the residues that are essential for binding to microtubules. We propose that functional microtubules and the dynein motor protein participate in transport of p53 and facilitate its accumulation in the nucleus after DNA damage.

publication date

  • October 2000

Research

keywords

  • Academic Article

Identity

Language

  • eng

Digital Object Identifier (DOI)

  • 10.1038/35036335

PubMed ID

  • 11025661

Additional Document Info

start page

  • 709

end page

  • 17

volume

  • 2

number

  • 10