Circulating human B cells that express surrogate light chains and edited receptors Academic Article uri icon

Overview

MeSH Major

  • B-Lymphocytes
  • Immunoglobulin Light Chains
  • RNA Editing

abstract

  • Immunoglobulin gene recombination can result in the assembly of self-reactive antibodies. Deletion, anergy or receptor editing normally silence B cells that produce these autoantibodies. Receptor editing is highly efficient in mouse B cells that carry pre-recombined autoantibody transgenes or gene "knock-ins". However, it has been difficult to identify cells that have edited receptors in unmanipulated mice and humans. To try to identify such cells we isolated and characterized B cells that coexpress surrogate and conventional light chains (V-preB+L+) from the blood of normal human donors. V-preB+L+ B cells express RAG mRNA, display an unusual heavy and light chain antibody repertoire consistent with antiself reactivity, and show evidence of receptor editing. These cells accumulate in the joints of patients with rheumatoid arthritis, consistent with a role for V-preB+L+ B cells and receptor editing in autoimmune disease.

publication date

  • September 2000

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed ID

  • 10973277

Additional Document Info

start page

  • 207

end page

  • 13

volume

  • 1

number

  • 3