The catalytic pathway of cytochrome P450cam at atomic resolution Academic Article Article uri icon

Overview

MeSH Major

  • National Institutes of Health (U.S.)
  • Translational Medical Research

abstract

  • Members of the cytochrome P450 superfamily catalyze the addition of molecular oxygen to nonactivated hydrocarbons at physiological temperature-a reaction that requires high temperature to proceed in the absence of a catalyst. Structures were obtained for three intermediates in the hydroxylation reaction of camphor by P450cam with trapping techniques and cryocrystallography. The structure of the ferrous dioxygen adduct of P450cam was determined with 0.91 angstrom wavelength x-rays; irradiation with 1.5 angstrom x-rays results in breakdown of the dioxygen molecule to an intermediate that would be consistent with an oxyferryl species. The structures show conformational changes in several important residues and reveal a network of bound water molecules that may provide the protons needed for the reaction.

publication date

  • March 3, 2000

Research

keywords

  • Academic Article

Identity

Digital Object Identifier (DOI)

  • 10.1126/science.287.5458.1615

PubMed ID

  • 10698731

Additional Document Info

start page

  • 1615

end page

  • 22

volume

  • 287

number

  • 5458