Approaching translation at atomic resolution. Review uri icon

Overview

MeSH

  • Models, Molecular
  • Protein Conformation

MeSH Major

  • Protein Biosynthesis
  • Ribosomes

abstract

  • Atomic resolution structures of 50S and 30S ribosomal particles have recently been solved by X-ray diffraction. These ribosomal structures show often unusual folds of ribosomal RNAs and proteins, and provide molecular explanations for fundamental aspects of translation. In the 50S structure, the active site for peptide bond formation was localized and found to consist of RNA. The ribosome is thus a ribozyme. In the 30S structures, tRNA binding sites were located, and molecular mechanisms for ribosomal fidelity were proposed. The 30S subunit particle has three globular domains, and relative movements of these domains may be required for translocation of the ribosome during protein synthesis. The structures are consistent with and rationalize decades of biochemical analysis of translation and usher in a molecular age in understanding the ribosome.

publication date

  • October 2000

has subject area

  • Models, Molecular
  • Protein Biosynthesis
  • Protein Conformation
  • Ribosomes

Research

keywords

  • Journal Article
  • Review

Identity

Language

  • eng

Digital Object Identifier (DOI)

  • 10.1038/79603

PubMed ID

  • 11017192

Additional Document Info

start page

  • 855

end page

  • 861

volume

  • 7

number

  • 10