Real-time measurements of vesicle-SNARE recycling in synapses of the central nervous system Academic Article uri icon

Overview

MeSH Major

  • Hippocampus
  • Membrane Proteins
  • Neurons
  • Synaptic Vesicles
  • Vesicular Transport Proteins

abstract

  • Following the fusion of synaptic vesicles with the presynaptic plasma membrane of nerve terminals by the process of exocytosis, synaptic-vesicle components are recycled to replenish the vesicle pool. Here we use a pH-sensitive green fluorescent protein to measure the residence time of VAMP, a vesicle-associated SNARE protein important for membrane fusion, on the surfaces of synaptic terminals of hippocampal neurons following exocytosis. The time course of VAMP retrieval depends linearly on the amount of VAMP that is added to the plasma membrane, with retrieval occurring between about 4 seconds and 90 seconds after exocytosis, and newly internalized vesicles are rapidly acidified. These data are well described by a model in which endocytosis appears to be saturable, but proceeds with an initial maximum velocity of about one vesicle per second. We also find that, following exocytosis, a portion of the newly inserted VAMP appears on the surface of the axon.

publication date

  • January 2000

Research

keywords

  • Academic Article

Identity

Language

  • eng

Digital Object Identifier (DOI)

  • 10.1038/35008615

PubMed ID

  • 10783237

Additional Document Info

start page

  • 197

end page

  • 204

volume

  • 2

number

  • 4