Dendritic cells genetically modified to express CD40 ligand and pulsed with antigen can initiate antigen-specific humoral immunity independent of CD4+ T cells. Academic Article uri icon

Overview

MeSH

  • Animals
  • B-Lymphocytes
  • Coculture Techniques
  • Cytokines
  • Female
  • Genetic Engineering
  • Genetic Vectors
  • Immunization
  • Mice
  • Mice, Inbred C57BL
  • Pseudomonas Infections
  • Pseudomonas aeruginosa

MeSH Major

  • CD4-Positive T-Lymphocytes
  • CD40 Ligand
  • Dendritic Cells

abstract

  • We have investigated whether dendritic cells genetically modified to express CD40 ligand and pulsed with antigen can trigger B cells to produce antigen-specific antibodies without CD4+ T-cell help. Dendritic cells modified with a recombinant adenovirus vector to express CD40 ligand and pulsed with heat-killed Pseudomonas induced naive B cells to produce antibodies against Pseudomonas in the absence of CD4+ T cells in vitro, initiated Pseudomonas-specific humoral immune responses in vivo in wild-type and CD4-/- mice, and protected immunized wild-type and CD4-/-, but not B-cell -/- mice, from lethal intrapulmonary challenge with Pseudomonas. Thus, genetic modification of dendritic cells with CD40 ligand enables them to present a complex mixture of microbial antigens and establish CD4+ T cell-independent, B cell-mediated protective immunity against a specific microbe.

publication date

  • October 2000

has subject area

  • Animals
  • B-Lymphocytes
  • CD4-Positive T-Lymphocytes
  • CD40 Ligand
  • Coculture Techniques
  • Cytokines
  • Dendritic Cells
  • Female
  • Genetic Engineering
  • Genetic Vectors
  • Immunization
  • Mice
  • Mice, Inbred C57BL
  • Pseudomonas Infections
  • Pseudomonas aeruginosa

Research

keywords

  • Journal Article

Identity

Language

  • eng

Digital Object Identifier (DOI)

  • 10.1038/80498

PubMed ID

  • 11017148

Additional Document Info

start page

  • 1154

end page

  • 1159

volume

  • 6

number

  • 10