Cloning genes encoding MHC class II-restricted antigens: Mutated CDC27 as a tumor antigen Academic Article Article uri icon


MeSH Major

  • Immunity
  • Neoplasms
  • Toll-Like Receptors


  • In an effort to identify tumor-specific antigens recognized by CD4(+) T cells, an approach was developed that allows the screening of an invariant chain-complementary DNA fusion library in a genetically engineered cell line expressing the essential components of the major histocompatibility complex (MHC) class II processing and presentation pathway. This led to the identification of a mutated form of human CDC27, which gave rise to an HLA-DR4-restricted melanoma antigen. A mutated form of triosephosphate isomerase, isolated by a biochemical method, was also identified as an HLA-DR1-restricted antigen. Thus, this approach may be generally applicable to the identification of antigens recognized by CD4(+) T cells, which could aid the development of strategies for the treatment of patients with cancer, autoimmune diseases, or infectious diseases.

publication date

  • May 21, 1999



  • Academic Article


Digital Object Identifier (DOI)

  • 10.1126/science.284.5418.1351

PubMed ID

  • 10334988

Additional Document Info

start page

  • 1351

end page

  • 4


  • 284


  • 5418