Vezf1: A Zn finger transcription factor restricted to endothelial cells and their precursors. Academic Article uri icon

Overview

MeSH

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Chromosome Mapping
  • DNA Primers
  • DNA, Complementary
  • DNA-Binding Proteins
  • Female
  • Gene Expression Regulation, Developmental
  • Humans
  • In Situ Hybridization
  • Kruppel-Like Transcription Factors
  • Male
  • Mice
  • Mice, Transgenic
  • Molecular Sequence Data
  • Molecular Weight
  • Stem Cells

MeSH Major

  • Endothelium, Vascular
  • Transcription Factors
  • Zinc Fingers

abstract

  • Using retroviral entrapment vectors, we identified a novel mouse gene whose expression is restricted to vascular endothelial cells and their precursors in the yolk sac blood islands. A 3.68-kb cDNA corresponding to the endogenous transcript was isolated using genomic DNA flanking the entrapment vector insertion as a probe. We have named this gene Vezf1 for vascular endothelial zinc finger 1. Vezf1 encodes a protein with a predicted molecular mass of 56 kDa and that contains six putative zinc finger domains and shows high homology to a previously identified human gene, DB1, that is believed to be involved in regulating expression of cytokine genes such as interleukin-3. In situ hybridization analysis revealed the onset of expression in advanced primitive streak-stage embryos being located in the extraembryonic mesodermal component of the visceral yolk sac and in the anteriormost mesoderm of the embryo proper. During head-fold and somite stages, expression was restricted to vascular endothelial cells that arise during both vasculogenesis and angiogenesis. Vezf1-related sequences were found to be highly conserved among higher vertebrate species that have acquired extraembryonic yolk sac membranes during evolution. The Vezf1 locus mapped to the proximal part of mouse chromosome 2, a region which has homology to human chromosome 9q. Vezf1 expression correlates temporally and spatially with the early differentiation of angioblasts into the endothelial cell lineage and the proliferation of endothelial cells of the embryonic vascular system. Thus, Vezf1 may play an important role in the endothelial lineage determination and may have an additional role during later stages of embryonic vasculogenesis and angiogenesis. Copyright 1999 Academic Press.

publication date

  • February 15, 1999

has subject area

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Chromosome Mapping
  • DNA Primers
  • DNA, Complementary
  • DNA-Binding Proteins
  • Endothelium, Vascular
  • Female
  • Gene Expression Regulation, Developmental
  • Humans
  • In Situ Hybridization
  • Kruppel-Like Transcription Factors
  • Male
  • Mice
  • Mice, Transgenic
  • Molecular Sequence Data
  • Molecular Weight
  • Stem Cells
  • Transcription Factors
  • Zinc Fingers

Research

keywords

  • Journal Article

Identity

Language

  • eng

Digital Object Identifier (DOI)

  • 10.1006/dbio.1998.9144

PubMed ID

  • 9986727

Additional Document Info

start page

  • 123

end page

  • 141

volume

  • 206

number

  • 2