Nuclear trafficking of Cubitus interruptus in the transcriptional regulation of Hedgehog target gene expression. Academic Article uri icon

Overview

MeSH

  • Animals
  • Cell Line
  • Genes, Reporter
  • Hedgehog Proteins
  • Kinetics
  • Polymerase Chain Reaction
  • Recombinant Proteins
  • Restriction Mapping
  • Signal Transduction
  • Transcription Factors
  • Transfection
  • Zinc Fingers

MeSH Major

  • Cell Nucleus
  • DNA-Binding Proteins
  • Drosophila
  • Drosophila Proteins
  • Gene Expression Regulation
  • Insect Proteins
  • Transcription, Genetic

abstract

  • Transcriptional activation of Hedgehog (Hh) target genes requires Cubitus interruptus, a 155 kDa cytoplasmic zinc finger protein (Ci155), which in the absence of Hh signaling is processed to form a nuclear repressor (Ci75). We show that Hh signaling reduces phosphorylation of Ci155, and this reduction in turn appears to decrease processing. Blocking processing with proteasome inhibitors or altered Ci proteins, however, is insufficient for activation of Hh targets. We find that Hh signaling increases the rate of Ci155 nuclear import, resulting in significant nuclear accumulation. Even in the absence of signaling, nuclear accumulation of Ci155 suffices for significant induction of Hh targets, and active nuclear export of Ci155 is an essential mechanism for maintenance of the unstimulated state.

publication date

  • August 6, 1999

has subject area

  • Animals
  • Cell Line
  • Cell Nucleus
  • DNA-Binding Proteins
  • Drosophila
  • Drosophila Proteins
  • Gene Expression Regulation
  • Genes, Reporter
  • Hedgehog Proteins
  • Insect Proteins
  • Kinetics
  • Polymerase Chain Reaction
  • Recombinant Proteins
  • Restriction Mapping
  • Signal Transduction
  • Transcription Factors
  • Transcription, Genetic
  • Transfection
  • Zinc Fingers

Research

keywords

  • Journal Article

Identity

Language

  • eng

PubMed ID

  • 10458606

Additional Document Info

start page

  • 305

end page

  • 316

volume

  • 98

number

  • 3