α(1,3)-Fucosyltransferase VII and α(2,3)-sialyltransferase IV are up- regulated in activated CD4 T cells and maintained after their differentiation into Th1 and migration into inflammatory sites Academic Article Article uri icon

Overview

MeSH Major

  • Host-Pathogen Interactions
  • Immunity, Innate
  • Infection
  • Receptors, Pattern Recognition

abstract

  • Activated Th1 CD4 T cells bind to P-selectin and migrate into inflamed tissue, whereas Th2 cells do not. We show that alpha(1, 3)-fucosyltransferase VII (FucT-VII) and alpha(2, 3)-sialyltransferase IV (ST3GalIV), which are crucial for the biosynthesis of functional P-selectin ligands, are absent in naive CD4 T cells, but are rapidly up-regulated upon activation. Th1 or Th2 differentiation in the presence of polarizing cytokines leads to down-regulation of FucT-VII mRNA selectively in Th2 but not in Th1 cells. Influencing the differentiation by varying the priming dose of antigenic peptide results in similar FucT-VII down-regulation only in Ag-specific Th2 cells. ST3GalIV levels remain elevated. FucT-VII and ST3GalIV mRNAs are also up-regulated by Th1 cells primed in vivo and recruited into the lymph nodes draining delayed-type hypersensitivity sites. We identify FucT-VII gene expression as a principal difference between Th1 and Th2 cells, and underscore the importance of FucT-VII and ST3GalIV expression for the biosynthesis of functional selectin ligands.

publication date

  • October 1999

Research

keywords

  • Academic Article

Identity

PubMed ID

  • 10490970

Additional Document Info

start page

  • 3746

end page

  • 52

volume

  • 163

number

  • 7