Developmental regulation of TCRδ locus accessibility and expression by the TCRδ enhancer Academic Article Article uri icon

Overview

MeSH Major

  • Cell Cycle Proteins
  • DNA-Activated Protein Kinase
  • DNA-Binding Proteins
  • Protein-Serine-Threonine Kinases
  • Recombination, Genetic
  • Tumor Suppressor Proteins

abstract

  • We have used gene-targeted mutation to assess the role of the T cell receptor delta (TCR delta) enhancer (E delta) in alphabeta and gammadelta T cell development. Mice lacking E delta exhibited no defects in alphabeta T cell development but had a severe reduction in thymic and peripheral gammadelta T cells and decreased VDJ delta rearrangements. Simultaneous deletion of both E delta and the TCR alpha enhancer (E alpha) demonstrated that residual TCR delta rearrangements were not driven by E alpha, implicating additional elements in TCR delta locus accessibility. Surprisingly, while deletion of E delta severely impaired germline TCR delta expression in double-negative thymocytes, absence of E delta did not affect expression of mature delta transcripts in gammadelta T cells. We conclude that E delta has an important role in TCR delta locus regulation at early, but not late, stages of gammadelta T cell development.

publication date

  • May 1999

Research

keywords

  • Academic Article

Identity

Digital Object Identifier (DOI)

  • 10.1016/S1074-7613(00)80050-3

PubMed ID

  • 10367896

Additional Document Info

start page

  • 503

end page

  • 13

volume

  • 10

number

  • 5