Intra-cluster recombination and var transcription switches in the antigenic variation of Plasmodium falciparum. Academic Article uri icon

Overview

MeSH

  • Animals
  • Erythrocytes
  • Gene Expression Regulation
  • Genes, Switch
  • Multigene Family
  • Promoter Regions, Genetic
  • Reverse Transcriptase Polymerase Chain Reaction

MeSH Major

  • Antigenic Variation
  • Genes, Protozoan
  • Plasmodium falciparum
  • Recombination, Genetic
  • Transcription, Genetic

abstract

  • Antigenic variation and immune evasion by Plasmodium falciparum parasitized erythrocytes are mediated by expression switches among members of the multicopy var gene family. Here we describe a cluster of var genes on chromosome 12 that showed spontaneous recombination and switches in the transcription of individual genes. The transcription switches were not associated with sequence changes in promoter regions. Transfected episomes containing a luciferase reporter under control of a var promoter were expressed regardless of the transcriptional status of the endogenous promoter. The results suggest epigenetic regulation of P. falciparum var gene transcription that depends upon the local structure of chromatin and its associated proteins.

publication date

  • June 25, 1999

has subject area

  • Animals
  • Antigenic Variation
  • Erythrocytes
  • Gene Expression Regulation
  • Genes, Protozoan
  • Genes, Switch
  • Multigene Family
  • Plasmodium falciparum
  • Promoter Regions, Genetic
  • Recombination, Genetic
  • Reverse Transcriptase Polymerase Chain Reaction
  • Transcription, Genetic

Research

keywords

  • Journal Article

Identity

Language

  • eng

PubMed ID

  • 10413047

Additional Document Info

start page

  • 107

end page

  • 116

volume

  • 101

number

  • 1-2