Curcumin inhibits cyclooxygenase-2 transcription in bile acid- and phorbol ester-treated human gastrointestinal epithelial cells Academic Article uri icon

Overview

MeSH Major

  • Chenodeoxycholic Acid
  • Curcumin
  • Cyclooxygenase Inhibitors
  • Digestive System
  • Isoenzymes
  • Prostaglandin-Endoperoxide Synthases
  • Tetradecanoylphorbol Acetate
  • Transcription, Genetic

abstract

  • We investigated whether curcumin, a chemopreventive agent, inhibited chenodeoxycholate (CD)- or phorbol ester (PMA)-mediated induction of cyclooxygenase-2 (COX-2) in several gastrointestinal cell lines (SK-GT-4, SCC450, IEC-18 and HCA-7). Treatment with curcumin suppressed CD- and PMA-mediated induction of COX-2 protein and synthesis of prostaglandin E2. Curcumin also suppressed the induction of COX-2 mRNA by CD and PMA. Nuclear run-offs revealed increased rates of COX-2 transcription after treatment with CD or PMA and these effects were inhibited by curcumin. Treatment with CD or PMA increased binding of AP-1 to DNA. This effect was also blocked by curcumin. In addition to the above effects on gene expression, we found that curcumin directly inhibited the activity of COX-2. These data provide new insights into the anticancer properties of curcumin.

publication date

  • March 15, 1999

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed ID

  • 10190560

Additional Document Info

start page

  • 445

end page

  • 51

volume

  • 20

number

  • 3