Hypophysectomy enhances interleukin-1β, tumor necrosis factor-α, and interleukin-10 mRNA expression in the rat brain Academic Article uri icon


MeSH Major

  • Brain
  • Interleukin-1
  • Interleukin-10
  • Pituitary Gland
  • RNA, Messenger
  • Tumor Necrosis Factor-alpha


  • Although the effects of various cytokines as regulators of hormone synthesis and production are well documented, the role for pituitary hormones as modulators of cytokine synthesis is not fully understood. In this study, we investigated the effect of pituitary hormones' depletion on cytokine synthesis after short- (21 days) and long- (35 days) term hypophysectomy (ST-HX and LT-HX, respectively). The expresssion of the proinflammatory cytokine interleukin-1beta (IL-1beta) and tumor necrosis factor-alpha (TNF-alpha) and the anti-inflammatory cytokines IL-10 and transforming growth factor-beta (TGF-beta) in the rat brain was studied using in situ hybridization. Our results indicate that IL-1beta mRNA-expressing cells were significantly upregulated at day 21 in hypophysectomized rats compared to sham-operated controls. This enhanced expression was also detected later at day 35 post hypophysectomy. However, TNF-alpha mRNA expression was significantly increased only at the later sampling interval. IL-10 mRNA-expressing cells were increased after long-term hypophysectomy compared to controls. TGF-beta mRNA-expressing cells were not increased after hypophysectomy. In conclusion, these results suggest a role for pituitary hormones in IL-1beta, TNF-alpha, and IL-10 synthesis.

publication date

  • July 27, 1999



  • Academic Article



  • eng

Digital Object Identifier (DOI)

  • 10.1089/107999099313712

PubMed ID

  • 10433358

Additional Document Info

start page

  • 583

end page

  • 7


  • 19


  • 6