Glutathione aerosol suppresses lung epithelial surface inflammatory cell-derived oxidants in cystic fibrosis. Academic Article uri icon

Overview

MeSH

  • Adult
  • Aerosols
  • Epithelial Cells
  • Female
  • Glutathione Disulfide
  • Humans
  • Inflammation
  • Male
  • Mononuclear Phagocyte System
  • Superoxides

MeSH Major

  • Antioxidants
  • Cystic Fibrosis
  • Glutathione
  • Lung
  • Oxidants

abstract

  • Cystic fibrosis (CF) is characterized by accumulation of activated neutrophils and macrophages on the respiratory epithelial surface (RES); these cells release toxic oxidants, which contribute to the marked epithelial derangements seen in CF. These deleterious consequences are magnified, since reduced glutathione (GSH), an antioxidant present in high concentrations in normal respiratory epithelial lining fluid (ELF), is deficient in CF ELF. To evaluate the feasibility of increasing ELF GSH levels and enhancing RES antioxidant protection, GSH aerosol was delivered (600 mg twice daily for 3 days) to seven patients with CF. ELF total, reduced, and oxidized GSH increased (P < 0.05, all compared with before GSH therapy), suggesting adequate RES delivery and utilization of GSH. Phorbol 12-myristate 13-acetate-stimulated superoxide anion (O2-.) release by ELF inflammatory cells decreased after GSH therapy (P < 0.002). This paralleled observations that GSH added in vitro to CF ELF inflammatory cells suppressed O2-. release (P < 0.001). No adverse effects were noted during treatment. Together, these observations demonstrate the feasibility of using GSH aerosol to restore RES oxidant-antioxidant balance in CF and support the rationale for further clinical evaluation.

publication date

  • July 1999

has subject area

  • Adult
  • Aerosols
  • Antioxidants
  • Cystic Fibrosis
  • Epithelial Cells
  • Female
  • Glutathione
  • Glutathione Disulfide
  • Humans
  • Inflammation
  • Lung
  • Male
  • Mononuclear Phagocyte System
  • Oxidants
  • Superoxides

Research

keywords

  • Journal Article

Identity

Language

  • eng

PubMed ID

  • 10409605

Additional Document Info

start page

  • 438

end page

  • 443

volume

  • 87

number

  • 1