Dissociation of basal turnover and cytokine-induced transcript stabilization of the human Cyclooxygenase-2 mRNA by mutagenesis of the 3@?-untranslated region Academic Article uri icon

Overview

MeSH Major

  • Cytokines
  • Isoenzymes
  • Mutagenesis
  • Prostaglandin-Endoperoxide Synthases
  • RNA, Messenger

abstract

  • The immediate early gene cyclooxygenase-2 (Cox-2), which encodes the inducible prostaglandin synthase enzyme, is regulated at the level of post-transcriptional mRNA turnover. In this study, the functional role of the 3'-untranslated region (3'-UTR) of the human Cox-2 gene was characterized. Deletion of the distal region of the 3'-UTR strongly inhibited basal mRNA turnover, suggesting that this region contains mRNA instability determinants. However, deletion of the proximal highly-conserved region (CR1: 6082-6198) resulted in increased basal turnover, indicating that it determines mRNA stability. All of the 3'-UTR constructs conferred IL-1-induced stabilization but not dexamethasone-induced down-regulation. Thus, distinct regions of the 3'-UTR of the Cox-2 transcript are involved in the regulation of basal and cytokine-induced mRNA metabolism.

publication date

  • January 26, 1998

Research

keywords

  • Academic Article

Identity

Language

  • eng

Digital Object Identifier (DOI)

  • 10.1006/bbrc.1997.7994

PubMed ID

  • 9464246

Additional Document Info

start page

  • 508

end page

  • 12

volume

  • 242

number

  • 3