Extracellular HIV-1 Tat protein induces a rapid and selective activation of protein kinase C (PKC)-α, -ε, and -ζ isoforms in PC12 cells Academic Article uri icon

Overview

MeSH Major

  • Diagnostic Techniques and Procedures
  • Laboratories
  • Magnetics

abstract

  • The addition in culture of extracellular HIV-1 Tat protein (0.1-1 nM) to PC12 cells induced a rapid increase of the bulk protein kinase C (PKC) catalytic activity. Among various PKC isoforms (alpha, beta I, beta II, delta, epsilon, eta, theta, and zeta) expressed in PC12 cells, Tat selectively stimulated alpha, epsilon, and zeta, as judged by activities in immunoprecipitates. Activation of these isoforms was suppressed by the tyrosine kinase inhibitor genistein. Moreover, PKC-zeta showed the fastest kinetics of activation in response to Tat, but PKC-alpha and PKC-epsilon showed the highest levels of activation. PKC-alpha activation was accompanied by a rise of intracellular IP3, while the PI 3-kinase inhibitors wortmannin and LY294002 suppressed PKC-epsilon activation. Taken together, these findings demonstrate that extracellular Tat shows a cytokine-like activity in PC12 cells, being able to trigger an intracellular signalling cascade which involves PKC-alpha, -epsilon, and -zeta.

publication date

  • January 14, 1998

Research

keywords

  • Academic Article

Identity

Digital Object Identifier (DOI)

  • 10.1006/bbrc.1997.7877

Additional Document Info

start page

  • 332

end page

  • 7

volume

  • 242

number

  • 2